I’ve been reading up on centriole biogenesis and centrosome duplication – look for more posts from myself on topics such as this: Plk4-Induced Centriole Biogenesis in Human Cells [Dev Cell. 2007 Aug;13(2):190-202]. From the introduction:
We have previously shown that overexpression of Plk4 in human cells causes the recruitment of electron-dense material onto the proximal walls of parental centrioles (Habedanck et al., 2005), suggesting that Plk4 is able to trigger procentriole formation. Here, we have used a cell line allowing the temporally controlled expression of Plk4 to study the formation of centrioles in human cells. We show that Plk4 triggers the simultaneous formation of multiple procentrioles around each pre-existing centriole. These multiple centrioles form during S phase and persist as flower-like structures throughout G2 and M phase before they disperse in response to disengagement during mitotic exit, giving rise to a typical centriole amplification phenotype. Through siRNA-mediated depletion of individual centrosomal proteins, we have identified several gene products important for Plk4-controlled centriole biogenesis and assigned individual proteins to distinct steps in the assembly pathway. Finally, we have been able to correlate these functional data with morphological analyses using immunoelectron microscopy. Taken together, these results provide a first molecular analysis of centriole formation in human cells.