Here’s an amazing video of cell divisions and movements in early embryonic zebrafish development:
Mo writes about the video and the science behind it at The Neurophilosophy Blog:
This reconstruction, produced by researchers from the European Molecular Biology Laboratory in Heidelberg, Germany using a technique called digital scanned laser light sheet fluorescence microscopy, shows the movements of all 16,000 cells in an 18-hour-old zebrafish embryo.
To make the film, the researchers injected a fluorescent protein into an embryo at the one cell stage. They began imaging at the 64-cell stage and captured images every 90 seconds for 12 hours.
This movie and 15 others accompany a paper published in a recent issue of Science.
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Not to mention the difficulties in learning new techniques quickly. Lately, I’ve been learning how to do RNA microinjections into 4-cell stage frog embryos. Simple, by the standards of your average embryologist, but my experience in embryology (or developmental biology, if you prefer) is limited – I was trained as a cell biologist. And learning experiences tend to involve me saying “I had better not do that again!” In other words, in the lab, I tend to learn by a winnowing process, until finally I’ve weeded out all the wrong ways to perform a technique. It’s not very efficient, but for cutting-edge protocols, one has to write the protocol themselves much of the time. The ancient Japanese proverb, “Fall down seven times, get up eight,” comes to mind.






